SIXTH AMENDMENT TO CONSTRUCTION LOAN AGREEMENT

Exhibit 10.1

SIXTH AMENDMENT TO CONSTRUCTION LOAN AGREEMENT

This Sixth Amendment to Construction Loan Agreement is dated as of the 23rd day of March, 2006, and is by and between DAKOTA ETHANOL, L.L.C., a South Dakota limited liability company (“BORROWER”) and FIRST NATIONAL BANK OF OMAHA (“BANK”), a national banking association established at Omaha, Nebraska.

WHEREAS, BANK and BORROWER executed a Construction Loan Agreement dated as of September 25, 2000 (the Construction Loan Agreement, together with all amendments thereto is herein called the “AGREEMENT”);

Now, therefore, for valuable consideration, receipt and adequacy of which is acknowledged, the parties agree as follows:

1.             All capitalized terms herein that are not otherwise defined shall have the meanings assigned to them in the AGREEMENT.

2.             BORROWER acknowledges and agrees it owes BANK (as part of its OBLIGATIONS):

•                  TERM NOTE 2 which has a current principal balance of $9,360,401.39

•                  TERM NOTE 4 which has a current principal balance of $88,121.03 that is to be paid on April 1, 2006;

•                  TERM NOTE 5 which has a current principal balance of $0, and a commitment of $5,000,000;

•                  REVOLVING NOTE which has a current principal balance of $0, and a commitment of $3,000,000.00.

3.             The parties desire to revise TERM NOTE 5 to amend it from a Reducing Revolver to a Long Term Revolving Note, which will provide at all times a $5,000,000 revolving facility to BORROWER after TERM NOTE 4 is paid, and to revise the repayment schedule of TERM NOTE 5.

4.             Attached hereto as Exhibit 6-A is a replacement TERM NOTE 5, which by execution hereof, will replace the TERM NOTE 5 Reducing Revolver with a new TERM NOTE 5 Long Term Revolver, with the revised repayment terms as described in the new TERM NOTE 5.

5.             Section 1.17 of the AGREEMENT (definition of MANAGEMENT CONTRACT) is hereby deleted in its entirety, effective immediately.

6.             Section 1.18 of the AGREEMENT is hereby amended to read, effective immediately:

1.18 “MARKETING CONTRACTS” means the written contract dated as of November 30, 2005, between BORROWER and Renewable Products Marketing